Lawrence, KS - Chemistry graduate student, Mohammed Al-Naqshabandi (Dr. Weis group), has been selected to receive the Student Research Competition Presentation Award. The award will be presented at the 2015 Graduate Student Awards Ceremony on Thursday, April 23rd from 3:30 - 5:00 p.m. at the Ballroom of the Kansas Union.
"The novel aspect of this research is to explore the capability of fast photochemical oxidation of protein (FPOP)- mass spectrometry analysis to probe intrinsically disordered proteins and their folded complexes." - Mohammed Al-Naqshabandi
Summary of the research:
Intrinsically disordered proteins (IDPs) have regions that are highly flexible and lack stable secondary or tertiary structure. Although many studies have shown that IDPs participate in functional interactions, less is known about the structural details of the interactions. Our previous work showed that hydrogen/deuterium exchange (HDX) can provide important information about IDPs, but labeling takes place on the millisecond-second timescale. Since protein folding is slower than microseconds, fast photochemical oxidation of proteins (FPOP) might reveal states of IDPs that are undetectable by HDX. Here, we evaluate the application of FPOP for characterizing IDPs. We are using two disordered proteins as a model system that co-fold upon binding.