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Analytical Seminars

Monday, 3:30 pm, 1003 Malott (unless specified)

Date Presenter / Topic
August 21 No seminar
August 28 Nilan Kamathewatta, Berrie Research Group
Development of Gold Nano Structures for Selective Immobilization of Proteins for Biosensing
September 4 No seminar (Labor Day)
September 11 Kelci Schilly, Lunte Research Group
Bioanalytical applications of microchip electrophoresis: Looking for life and things that destroy it
September 18 Milani Patabandige, Desaire Research Group
Development of an Efficient Analysis Approach for N-glycosylation Site Profiling of Heavily Glycosylated Proteins
September 25 Charuni Amarasekara, Soper Research Group
Electrokinetic Transport of Deoxynucleotide Monophosphates (dNMPs) Through Polymer Nanochannels: Identifying Mononucleotides via their Time-of-Flight for Single Molecule DNA Sequencing
October 2 No seminar
October 9 Yuqi Shi, Weis Research Group
Sparse Representation of Hydrogen Exchange Mass Spectrometry (HX-MS) Data by Using LASSO
October 16 No seminar (Fall Break)
October 23 Kevin Leonard, Ph.D., Assistant Professor
KU Dept. of Chemical & Petroleum Engineering
Center for Environmentally Beneficial Catalysis
Characterizing Electrocatalysts With Scanning Electrochemical Microscopy
Abstract
Scanning electrochemical microscopy (SECM) is an imaging technique capable of providing chemical and topographic information, especially about surfaces immersed in a solution. It is based on moving a small tip electrode very close to a substrate surface. Unlike optical methods, it is unfettered by diffraction limits, and it does not require high energy or intense irradiation. In this talk, we will discuss using SECM to understand reaction mechanisms and active site densities of electrocatalysts for the water-splitting and CO2 reduction reactions. In addition, we will discuss a new nanoscale imaging mode of SECM used to understand the relationship between the structure and the reactivity of electrocatalytic metal nanoparticles reacting in the solution phase.
October 30 No seminar
November 6 No seminar
November 13 Brandon DeKosky, Ph.D., Assistant Professor
KU Depts. of Chemical & Petroleum Engineering and Pharmaceutical Chemistry
Comprehensive analyses of the antibody response to vaccination and infection
Abstract
High-throughput sequencing of antibodies has emerged as a critical step in understanding of adaptive responses following infection, vaccination or in autoimmunity. However, determining native antibody variable region heavy:light pairs (VH:VL) remains a major challenge, and no technologies exist to adequately interrogate the >106 B cells in typical specimens. We developed a low-cost single-cell technology for sequencing complete antibody variable region repertoires from >5x106 B cells per experiment. Massive VH:VL repertoire analyses of human donors enabled rapid antibody discovery while also providing novel insights regarding the immune response in healthy individuals. Most recently, we developed a facile technology for determining functional antibody binding. In this workflow, gene libraries of natively paired variable region heavy and light (VH:VL) amplicons are produced en masse and expressed in a yeast display platform optimized for human antibody gene surface expression. The yeast library is then tested for binding to pathogen-derived proteins or peptides using flow cytometry. These projects have been applied for efficient isolation of HIV-1 broadly neutralizing antibodies (bNAbs) from the B cell repertoire of an HIV-1 slow progressor and high-affinity neutralizing antibodies targeting Ebola virus (EBOV) glycoprotein from a vaccinated donor. Additional studies related to Zika virus and HIV vaccine responses will also be discussed. As DNA sequencing and technologies continue to progress, low-cost high-throughput single-cell antibody sequencing approaches will enable rapid antibody discovery and provide new insights into humoral immune responses associated with vaccine development, autoimmunity, and protection against infectious diseases.
November 20 Juan Rincon, Weis Research Group
Study of a scFv as a protein switch and as a model to predict long term stability studies using hydrogen exchange mass spectrometry
November 27 Mei He, Ph.D., Assistant Professor
KU Depts. of Chemical & Petroleum Engineering and Chemistry (Spring 2018)
3D Microfabrication for Understanding Adaptive Immunity-Inspired Therapeutics
Abstract
Understanding the complexity and high adaptability of human immunity allows us to take advantages of nature's insights for overcoming current challenges imposed on therapeutic manufacturing systems. Such immunity intelligence harnesses the structure, function, and information in one ecosystem for self-learned, adaptive problem solving. Inspired by the human immunity which manipulates immune cells and organs to produce therapeutic solutions, we intend to mimic such bio-inspired manufacturing process for generating novel therapeutic solutions. This presentation introduces cutting-edge 3D printing and microfabrication for studying live cell manufactured extracellular vesicles and exosomes, which will have important implications for cancer immunotherapy.
December 4 No seminar

 


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